LYMPHOID NEOPLASIA Rational engineering of L-asparaginase reveals importance of dual activity for cancer cell toxicity
نویسندگان
چکیده
1Biomolecular Modelling Laboratory, Cancer Research UK London Research Institute, Lincoln’s Inn Fields Laboratories, London, United Kingdom; 2Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel; 3Department of Bioinformatics and Telemedicine, Jagiellonian University, Collegium Medicum, Krakow, Poland; and 4Cancer Research UK Children’s Cancer Group, School of Cancer and Enabling Sciences, Manchester Academic Health Science Centre & Central Manchester University Hospitals, National Health Service Foundation Trust, University of Manchester, Manchester, United Kingdom;
منابع مشابه
Rational engineering of L-asparaginase reveals importance of dual activity for cancer cell toxicity.
Using proteins in a therapeutic context often requires engineering to modify functionality and enhance efficacy. We have previously reported that the therapeutic antileukemic protein macromolecule Escherichia coli L-asparaginase is degraded by leukemic lysosomal cysteine proteases. In the present study, we successfully engineered L-asparaginase to resist proteolytic cleavage and at the same tim...
متن کاملValidation of a Simple and Rapid Method for Assessment of Intracellular Bacterial Asparaginase
L-Asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.In this study, simple and advantageous method has been validated for rapid and precise determination of intracellular L-Asparaginasein bacterial species. A suspension of bacterial cells was used instead ofcell extract and incubated by substrate (asparagine) after simple wash and centrifugati...
متن کاملL-Asparaginase-producing Rouxiella Species Isolation, Antileukemia Activity Evaluation, and Enzyme Production Optimization
Background: L-Asparaginase (L-Asp) is used as an efficient anti-cancer drug, especially for acute lymphoblastic leukemia (ALL). Currently, two bacterial asparaginase isoenzymes are used for cancer treatment. Therefore, this research focused on isolating native bacteria with the ability to produce L-Asp. Materials and methods: L-Asp producing bacteria were isolated from soil samples on 9K medi...
متن کاملL-Asparaginase Activity in Cell Lysates and Culture Media of Halophilic Bacterial Isolates
The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated the ability to gro...
متن کاملEffect of Conjugation of Activated Glutaraldehyde-Nanochitosan with L-Asparaginase as an Anti Cancer Enzyme on its Stability and Physicochemical Properties
Introduction: The bacterial Asparaginase is used in the treatment of asparagine-dependent tumors, particularly lymphatic sarcoma and acute lymphoblastic leukemia. However, the instability of the enzyme increases the number of injections that are accompanied by high immune responses. The aim of this study was to investigate the conjugation of L-asparaginase with nanochitosan glutaraldehyde (NCG)...
متن کامل